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Antibodies bound to the blocking peptide no longer bind for the epitope within the goal protein. By evaluating the staining through the blocked antibody compared to the antibody by itself, one can see which staining is specific.

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This guidebook outlines further attainable optimization of mobile and immunoassay parameters to make certain the very best outcomes are...

This tutorial outlines further attainable optimization of mobile and immunoassay parameters to ensure the best possible effects are...

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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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Blocking peptides are peptides that bind particularly towards the target antibody and block antibody binding. These peptide generally consists of the epitope recognized by the antibody.

Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a complex with and features to be a regulatory subunit of CDK2, whose action is required for cell cycle G1/S transition. This protein accumulates in the G1-S period boundary and is also degraded as cells development through S phase.

Overexpression of the gene has become observed in several tumors, which leads to chromosome instability, and therefore might lead to tumorigenesis. This protein was discovered to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant position in advertising mobile-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually described. Two extra splice variants were being documented but specific nucleotide sequence info isn't nevertheless offered.

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